› 3rwb

X-ray diffraction
1.7Å resolution

Crystal structure of complex of 4PAL (4-Pyridoxolactone) and PLDH (tetrameric pyridoxal 4-dehydrogenase) from Mesorhizobium loti

Released:
DOI: 10.2210/pdb3rwb/pdb
PDB entry 3rwb coloured by chain, front view.
PDB entry 3rwb coloured by chain, side view.
PDB entry 3rwb coloured by chain, top view.
Source organism: Mesorhizobium loti
Entry author: Chu HN

Function and Biology Details

Reaction catalysed: 
Pyridoxal + NAD(+) = 4-pyridoxolactone + NADH
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189125 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pyridoxal 4-dehydrogenase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 247 amino acids
Theoretical weight: 25.43 KDa
Source organism: Mesorhizobium loti
Expression system: Escherichia coli
UniProt:
  • Canonical: Q988B7 (Residues: 2-248; Coverage: 100%)
Gene names: mlr6807, pldh-t
Sequence domains: Enoyl-(Acyl carrier protein) reductase
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments


Cofactor: Ligand NAD 4 x NAD
2 bound ligands:
Ligand 4PL 4 x 4PL
Ligand GOL 5 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL38B1
Spacegroup: P21
Unit cell:
a: 85.537Å b: 50.211Å c: 94.266Å
α: 90° β: 90.47° γ: 90°
R-values:
R R work R free
0.136 0.135 0.166
Expression system: Escherichia coli

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