› 3s29

X-ray diffraction
2.85Å resolution

The crystal structure of sucrose synthase-1 from Arabidopsis thaliana and its functional implications.

Released:
DOI: 10.2210/pdb3s29/pdb
PDB entry 3s29 coloured by chain, front view.
PDB entry 3s29 coloured by chain, side view.
PDB entry 3s29 coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
The structure of sucrose synthase-1 from Arabidopsis thaliana and its functional implications.
Zheng Y, Anderson S, Zhang Y, Garavito RM
J Biol Chem 286 36108-36118 (2011)
PMID: 21865170

Function and Biology Details

Reaction catalysed: 
NDP-glucose + D-fructose = NDP + sucrose
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155847 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sucrose synthase 1 Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 816 amino acids
Theoretical weight: 94.18 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P49040 (Residues: 1-808; Coverage: 100%)
Gene names: At5g20830, SUS1, T1M15.230
Sequence domains:
Structure domains:

Ligands and Environments

5 bound ligands:
Ligand UDP 8 x UDP
Ligand FRU 8 x FRU
Ligand SO4 30 x SO4
Ligand MLA 12 x MLA
Ligand K 8 x K
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: C2
Unit cell:
a: 277.16Å b: 261.5Å c: 161.1Å
α: 90° β: 109.27° γ: 90°
R-values:
R R work R free
0.187 0.185 0.234
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

8 review citations

An Overview of Sucrose Synthases in Plants.
Stein et al. (2019)
7 more

5 mentions without citation

The structure of sucrose synthase-1 from Arabidopsis thaliana and its functional implications.
Zheng et al. (2011)
4 more