› 3sg8

X-ray diffraction
1.8Å resolution

Crystal Structure of Aminoglycoside-2''-Phosphotransferase Type IVa Tobramycin Complex

Released:
DOI: 10.2210/pdb3sg8/pdb
PDB entry 3sg8 coloured by chain, front view.
PDB entry 3sg8 coloured by chain, side view.
PDB entry 3sg8 coloured by chain, top view.
Source organism: Enterococcus casseliflavus
Primary publication:
Crystal structures of antibiotic-bound complexes of aminoglycoside 2''-phosphotransferase IVa highlight the diversity in substrate binding modes among aminoglycoside kinases.
Shi K, Houston DR, Berghuis AM
Biochemistry 50 6237-44 (2011)
PMID: 21678960

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-130603 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aminoglycoside phosphotransferase domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 304 amino acids
Theoretical weight: 35.84 KDa
Source organism: Enterococcus casseliflavus
Expression system: Escherichia coli
UniProt:
  • Canonical: O68183 (Residues: 1-297; Coverage: 99%)
Gene name: aph(2'')-Id
Sequence domains: Phosphotransferase enzyme family
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand TOY 2 x TOY
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: P21
Unit cell:
a: 43.334Å b: 101.479Å c: 73.455Å
α: 90° β: 100.78° γ: 90°
R-values:
R R work R free
0.188 0.185 0.242
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Resilience of biochemical activity in protein domains in the face of structural divergence.
Zhang et al. (2014)
2 more

11 mentions without citation

Strategies to overcome the action of aminoglycoside-modifying enzymes for treating resistant bacterial infections.
Labby et al. (2013)
10 more