› 3uom

X-ray diffraction
2.02Å resolution

Ca2+ complex of Human skeletal calsequestrin

Released:
DOI: 10.2210/pdb3uom/pdb
PDB entry 3uom coloured by chain, front view.
PDB entry 3uom coloured by chain, side view.
PDB entry 3uom coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
High-capacity Ca2+ binding of human skeletal calsequestrin.
Sanchez EJ, Lewis KM, Danna BR, Kang C
J Biol Chem 287 11592-601 (2012)
PMID: 22337878

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152006 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Calsequestrin-1 Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 362 amino acids
Theoretical weight: 41.72 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P31415 (Residues: 35-396; Coverage: 100%)
Gene names: CASQ, CASQ1
Sequence domains: Calsequestrin
Structure domains: Glutaredoxin

Ligands and Environments

3 bound ligands:
Ligand MRD 26 x MRD
Ligand MPD 16 x MPD
Ligand CA 86 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: P1
Unit cell:
a: 89.799Å b: 89.793Å c: 119.161Å
α: 90.13° β: 89.9° γ: 60.05°
R-values:
R R work R free
0.182 0.182 0.213
Expression system: Escherichia coli

Quick links

Citations

9 review citations

Ryanodine receptors: allosteric ion channel giants.
Van Petegem F. (2015)
8 more

12 mentions without citation

Critical Role of Intracellular RyR1 Calcium Release Channels in Skeletal Muscle Function and Disease.
Hernández-Ochoa et al. (2015)
11 more