› 3vrf

X-ray diffraction
1.55Å resolution

The crystal structure of hemoglobin from woolly mammoth in the carbonmonoxy forms

Released:
DOI: 10.2210/pdb3vrf/pdb
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PDB entry 3vrf coloured by chain, front view.
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PDB entry 3vrf coloured by chain, top view.
Source organism: Mammuthus primigenius
Primary publication:
Structures of haemoglobin from woolly mammoth in liganded and unliganded states.
Noguchi H, Campbell KL, Ho C, Unzai S, Park SY, Tame JR
Acta Crystallogr D Biol Crystallogr 68 1441-9 (2012)
PMID: 23090393

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-112556 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Globin domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 141 amino acids
Theoretical weight: 15.54 KDa
Source organism: Mammuthus primigenius
Expression system: Escherichia coli
UniProt:
  • Canonical: D3U1H8 (Residues: 2-142; Coverage: 99%)
Gene name: HBA-T2
Sequence domains: Globin
Structure domains: Globins
Globin domain-containing protein Chain: B
Molecule details ›
Chain: B
Length: 146 amino acids
Theoretical weight: 16.18 KDa
Source organism: Mammuthus primigenius
Expression system: Escherichia coli
UniProt:
  • Canonical: D3U1H9 (Residues: 2-147; Coverage: 99%)
Gene names: D, HBB
Sequence domains: Globin
Structure domains: Globins

Ligands and Environments

2 bound ligands:
Ligand HEM 2 x HEM
Ligand CMO 2 x CMO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-17A
Spacegroup: C2
Unit cell:
a: 109.486Å b: 61.79Å c: 53.57Å
α: 90° β: 110.35° γ: 90°
R-values:
R R work R free
0.172 0.171 0.199
Expression system: Escherichia coli

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Citations

4 review citations

New look at hemoglobin allostery.
Yuan et al. (2015)
3 more

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