› 3vvr

X-ray diffraction
3Å resolution

Crystal structure of MATE in complex with MaD5

Released:
DOI: 10.2210/pdb3vvr/pdb
Model geometry
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PDB entry 3vvr coloured by chain, front view.
PDB entry 3vvr coloured by chain, side view.
PDB entry 3vvr coloured by chain, top view.
Source organism: Pyrococcus furiosus
Primary publication:
Structural basis for the drug extrusion mechanism by a MATE multidrug transporter.
Tanaka Y, Hipolito CJ, Maturana AD, Ito K, Kuroda T, Higuchi T, Katoh T, Kato HE, Hattori M, Kumazaki K, Tsukazaki T, Ishitani R, Suga H, Nureki O
Nature 496 247-51 (2013)
PMID: 23535598

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-164189 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
MATE family efflux transporter Chain: A
Molecule details ›
Chain: A
Length: 461 amino acids
Theoretical weight: 49.28 KDa
Source organism: Pyrococcus furiosus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8U2X0 (Residues: 1-461; Coverage: 100%)
Gene name: PF0708
Sequence domains: MatE
macrocyclic peptide Chain: B
Molecule details ›
Chain: B
Length: 12 amino acids
Theoretical weight: 1.1 KDa
Source organism: Pyrococcus furiosus
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand OLC 3 x OLC
1 modified residue:
Ligand DPN 1 x DPN

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SPRING-8 BEAMLINE BL32XU
Spacegroup: C2
Unit cell:
a: 158.521Å b: 59.599Å c: 68.335Å
α: 90° β: 90.99° γ: 90°
R-values:
R R work R free
0.271 0.266 0.309
Expression systems:
  • Escherichia coli
  • Not provided

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Citations

54 review citations

The challenge of efflux-mediated antibiotic resistance in Gram-negative bacteria.
Li et al. (2015)
53 more

5 mentions without citation

A comprehensive review of the lipid cubic phase or in meso method for crystallizing membrane and soluble proteins and complexes.
Caffrey M. (2015)
4 more

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