› 3wqv

X-ray diffraction
2.04Å resolution

Crystal structure of Ostrinia furnacalis Group I chitinase catalytic domain in complex with a(GlcN)5

Released:
DOI: 10.2210/pdb3wqv/pdb
PDB entry 3wqv coloured by chain, front view.
PDB entry 3wqv coloured by chain, side view.
PDB entry 3wqv coloured by chain, top view.
Source organism: Ostrinia furnacalis
Primary publication:
Fully deacetylated chitooligosaccharides act as efficient glycoside hydrolase family 18 chitinase inhibitors.
Chen L, Zhou Y, Qu M, Zhao Y, Yang Q
J Biol Chem 289 17932-40 (2014)
PMID: 24828498

Function and Biology Details

Reaction catalysed: 
Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174088 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Chitinase Chain: A
Molecule details ›
Chain: A
Length: 395 amino acids
Theoretical weight: 45 KDa
Source organism: Ostrinia furnacalis
Expression system: Komagataella phaffii GS115
UniProt:
  • Canonical: Q2V6H4 (Residues: 19-407; Coverage: 73%)
Sequence domains: Glycosyl hydrolases family 18
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: GCS
Carbohydrate polymer
2 bound ligands:
Ligand NAG 2 x NAG
Ligand EPE 1 x EPE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P65
Unit cell:
a: 94.222Å b: 94.222Å c: 122.43Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.15 0.15 0.168
Expression system: Komagataella phaffii GS115

Quick links

Citations

1 review citation

A Review of the Preparation, Analysis and Biological Functions of Chitooligosaccharide.
Liang et al. (2018)
14 other articles

3 mentions without citation

Fully deacetylated chitooligosaccharides act as efficient glycoside hydrolase family 18 chitinase inhibitors.
Chen et al. (2014)
2 more