› 3wtk

X-ray diffraction
2.69Å resolution

Crystal Structure of Lymnaea stagnalis Acetylcholine-Binding Protein Q55R Mutant Complexed with Thiacloprid

Released:
DOI: 10.2210/pdb3wtk/pdb
PDB entry 3wtk coloured by chain, front view.
PDB entry 3wtk coloured by chain, side view.
PDB entry 3wtk coloured by chain, top view.
Source organism: Lymnaea stagnalis
Primary publication:
Studies on an acetylcholine binding protein identify a basic residue in loop G on the β1 strand as a new structural determinant of neonicotinoid actions.
Ihara M, Okajima T, Yamashita A, Oda T, Asano T, Matsui M, Sattelle DB, Matsuda K
Mol Pharmacol 86 736-46 (2014)
PMID: 25267717

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-157653 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Acetylcholine-binding protein Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 214 amino acids
Theoretical weight: 24.25 KDa
Source organism: Lymnaea stagnalis
Expression system: Komagataella pastoris
UniProt:
  • Canonical: P58154 (Residues: 21-229; Coverage: 100%)
Sequence domains: Neurotransmitter-gated ion-channel ligand binding domain
Structure domains: Neurotransmitter-gated ion-channel ligand-binding domain

Ligands and Environments

1 bound ligand:
Ligand TH4 5 x TH4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: P65
Unit cell:
a: 74.534Å b: 74.534Å c: 350.816Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.203 0.2 0.274
Expression system: Komagataella pastoris

Quick links

Citations

5 review citations

Structural Studies of Nicotinic Acetylcholine Receptors: Using Acetylcholine-Binding Protein as a Structural Surrogate.
Shahsavar et al. (2016)
4 more

2 mentions without citation

Loops D, E and G in the Drosophila Dα1 subunit contribute to high neonicotinoid sensitivity of Dα1-chicken β2 nicotinic acetylcholine receptor.
Ihara et al. (2018)
1 more