› 3x1g

X-ray diffraction
1.3Å resolution

H294M mutant of copper-containing nitrite reductase from Geobacillus thermodenitrificans showing two coordination geometries at the T2Cu site

Released:
DOI: 10.2210/pdb3x1g/pdb
PDB entry 3x1g coloured by chain, front view.
PDB entry 3x1g coloured by chain, side view.
PDB entry 3x1g coloured by chain, top view.
Source organism: Geobacillus thermodenitrificans NG80-2
Entry authors: Fukuda Y, Inoue T

Function and Biology Details

Reaction catalysed: 
Nitric oxide + H(2)O + ferricytochrome c = nitrite + ferrocytochrome c + 2 H(+)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-107338 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Copper-containing nitrite reductase Chain: A
Molecule details ›
Chain: A
Length: 352 amino acids
Theoretical weight: 38.42 KDa
Source organism: Geobacillus thermodenitrificans NG80-2
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A4IL26 (Residues: 1-352; Coverage: 100%)
Gene names: GTNG_0650, nirK
Sequence domains:
Structure domains: Cupredoxins - blue copper proteins

Ligands and Environments

3 bound ligands:
Ligand CU 6 x CU
Ligand ACY 2 x ACY
Ligand MPD 1 x MPD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL38B1
Spacegroup: R3
Unit cell:
a: 115.275Å b: 115.275Å c: 84.691Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.107 0.106 0.128
Expression system: Escherichia coli BL21(DE3)

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