› 3ziw

X-ray diffraction
1.9Å resolution

Clostridium perfringens enterotoxin, D48A mutation and N-terminal 37 residues deleted

Released:
DOI: 10.2210/pdb3ziw/pdb
PDB entry 3ziw coloured by chain, front view.
PDB entry 3ziw coloured by chain, side view.
PDB entry 3ziw coloured by chain, top view.
Source organism: Clostridium perfringens
Primary publication:
Structure of a C. perfringens enterotoxin mutant in complex with a modified Claudin-2 extracellular loop 2.
Yelland TS, Naylor CE, Bagoban T, Savva CG, Moss DS, McClane BA, Blasig IE, Popoff M, Basak AK
J Mol Biol 426 3134-3147 (2014)
PMID: 25020226

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-134388 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Heat-labile enterotoxin B chain Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 286 amino acids
Theoretical weight: 31.48 KDa
Source organism: Clostridium perfringens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P01558 (Residues: 38-319; Coverage: 88%)
Gene name: cpe
Sequence domains: Clostridium enterotoxin
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand P6G 14 x P6G
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: C2
Unit cell:
a: 190.66Å b: 128.02Å c: 136.43Å
α: 90° β: 133.81° γ: 90°
R-values:
R R work R free
0.176 0.175 0.196
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Pore-forming toxins: ancient, but never really out of fashion.
Dal Peraro et al. (2016)
3 more

5 mentions without citation

Structural Basis of the Pore-Forming Toxin/Membrane Interaction.
Li et al. (2021)
4 more