› 4he2

X-ray diffraction
1.6Å resolution

Crystal structure of human muscle fructose-1,6-bisphosphatase Q32R mutant complex with AMP

Released:
DOI: 10.2210/pdb4he2/pdb
PDB entry 4he2 coloured by chain, front view.
PDB entry 4he2 coloured by chain, side view.
PDB entry 4he2 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structures of human muscle fructose-1,6-bisphosphatase: novel quaternary states, enhanced AMP affinity, and allosteric signal transmission pathway.
Shi R, Chen ZY, Zhu DW, Li C, Shan Y, Xu G, Lin SX
OpenAccess logo PLoS One 8 e71242 (2013)
PMID: 24086250

Function and Biology Details

Reaction catalysed: 
D-fructose 1,6-bisphosphate + H(2)O = D-fructose 6-phosphate + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126382 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fructose-1,6-bisphosphatase isozyme 2 Chain: A
Molecule details ›
Chain: A
Length: 338 amino acids
Theoretical weight: 36.7 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O00757 (Residues: 2-339; Coverage: 100%)
Gene name: FBP2
Sequence domains:
Structure domains:

Ligands and Environments

5 bound ligands:
Ligand AMP 1 x AMP
Ligand PO3 1 x PO3
Ligand MG 3 x MG
Ligand ZN 1 x ZN
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X8C
Spacegroup: P42212
Unit cell:
a: 73.366Å b: 73.366Å c: 146.622Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.182 0.181 0.194
Expression system: Escherichia coli BL21(DE3)

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Citations

1 review citation

Diffuse axonal injury after traumatic cerebral microbleeds: an evaluation of imaging techniques.
Liu et al. (2014)
6 other articles

3 mentions without citation

A Review on Cyclodextrins/Estrogens Inclusion Complexes.
Araj et al. (2023)
2 more