› 4kty

X-ray diffraction
1.98Å resolution

Fibrin-stabilizing factor with a bound ligand

Released:
DOI: 10.2210/pdb4kty/pdb
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PDB entry 4kty coloured by chain, front view.
PDB entry 4kty coloured by chain, side view.
PDB entry 4kty coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of active coagulation factor XIII triggered by calcium binding: basis for the design of next-generation anticoagulants.
Stieler M, Weber J, Hils M, Kolb P, Heine A, Büchold C, Pasternack R, Klebe G
Angew Chem Int Ed Engl 52 11930-4 (2013)
PMID: 24115223

Function and Biology Details

Reaction catalysed: 
A protein-L-glutamine + a protein-L-lysine = a protein with an N(6)-(gamma-glutamyl)-L-lysine cross-link + NH(3)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132549 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Coagulation factor XIII A chain Chains: A, B
Molecule details ›
Chains: A, B
Length: 738 amino acids
Theoretical weight: 84.21 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P00488 (Residues: 2-732; Coverage: 100%)
Gene names: F13A, F13A1
Sequence domains:
Structure domains:
Peptide-like ligand Chains: C, D
Molecule details ›
Chains: C, D
Length: 9 amino acids
Theoretical weight: 1.04 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

3 bound ligands:
Ligand CA 6 x CA
Ligand GOL 9 x GOL
Ligand SO4 1 x SO4
3 modified residues:
Ligand DAS 2 x DAS
Ligand ABA 2 x ABA
Ligand NLE 2 x NLE

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: BESSY BEAMLINE 14.2
Spacegroup: P1
Unit cell:
a: 56.761Å b: 80.51Å c: 102.844Å
α: 88.14° β: 76.7° γ: 82.01°
R-values:
R R work R free
0.171 0.169 0.206
Expression systems:
  • Spodoptera frugiperda
  • Not provided

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Citations

3 review citations

Factor XIII-A: An Indispensable "Factor" in Haemostasis and Wound Healing.
Alshehri et al. (2021)
2 more

14 mentions without citation

Revisiting the mechanism of coagulation factor XIII activation and regulation from a structure/functional perspective.
Gupta et al. (2016)
13 more

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