› 4mat

X-ray diffraction
2Å resolution

E.COLI METHIONINE AMINOPEPTIDASE HIS79ALA MUTANT

Released:
DOI: 10.2210/pdb4mat/pdb
PDB entry 4mat coloured by chain, front view.
PDB entry 4mat coloured by chain, side view.
PDB entry 4mat coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Escherichia coli methionine aminopeptidase: implications of crystallographic analyses of the native, mutant, and inhibited enzymes for the mechanism of catalysis.
Lowther WT, Orville AM, Madden DT, Lim S, Rich DH, Matthews BW
Biochemistry 38 7678-88 (1999)
PMID: 10387007

Function and Biology Details

Reaction catalysed: 
Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142397 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Methionine aminopeptidase Chain: A
Molecule details ›
Chain: A
Length: 278 amino acids
Theoretical weight: 31.02 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AE18 (Residues: 1-264; Coverage: 100%)
Gene names: JW0163, b0168, map
Sequence domains: Metallopeptidase family M24
Structure domains: Creatinase/methionine aminopeptidase superfamily

Ligands and Environments

1 bound ligand:
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P21
Unit cell:
a: 38.648Å b: 68.298Å c: 55.449Å
α: 90° β: 105.98° γ: 90°
R-values:
R R work R free
0.184 not available not available
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Protein-reactive natural products.
Drahl et al. (2005)
5 more

2 mentions without citation

Do quality improvement collaboratives' educational components match the dominant learning style preferences of the participants?
Weggelaar-Jansen et al. (2015)
1 more