› 4wj8

X-ray diffraction
2.87Å resolution

Human Pyruvate Kinase M2 Mutant C424A

Released:
DOI: 10.2210/pdb4wj8/pdb
PDB entry 4wj8 coloured by chain, front view.
PDB entry 4wj8 coloured by chain, side view.
PDB entry 4wj8 coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Mitchell T, Yuan M, McNae I, Morgan H, Walkinshaw MD

Function and Biology Details

Reactions catalysed: 
ATP + pyruvate = ADP + phosphoenolpyruvate
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146985 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pyruvate kinase PKM Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 551 amino acids
Theoretical weight: 60.16 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P14618 (Residues: 1-531; Coverage: 100%)
Gene names: OIP3, PK2, PK3, PKM, PKM2
Sequence domains:
Structure domains:

Ligands and Environments

7 bound ligands:
Ligand OXD 4 x OXD
Ligand FBP 2 x FBP
Ligand K 4 x K
Ligand MG 4 x MG
Ligand GOL 5 x GOL
Ligand PO4 3 x PO4
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: P21
Unit cell:
a: 94.359Å b: 115.26Å c: 109.401Å
α: 90° β: 114.43° γ: 90°
R-values:
R R work R free
0.202 0.2 0.243
Expression system: Escherichia coli

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