› 4xz2

X-ray diffraction
2.67Å resolution

Human platelet phosphofructokinase in an R-state in complex with ADP and F6P, crystal form I

Released:
DOI: 10.2210/pdb4xz2/pdb
PDB entry 4xz2 coloured by chain, front view.
PDB entry 4xz2 coloured by chain, side view.
PDB entry 4xz2 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of human platelet phosphofructokinase-1 locked in an activated conformation.
Kloos M, Brüser A, Kirchberger J, Schöneberg T, Sträter N
Biochem J 469 421-32 (2015)
PMID: 26205495

Function and Biology Details

Reaction catalysed: 
ATP + beta-D-fructose 6-phosphate = ADP + beta-D-fructose 1,6-bisphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169633 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ATP-dependent 6-phosphofructokinase, platelet type Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 761 amino acids
Theoretical weight: 83.16 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q01813 (Residues: 26-762; Coverage: 94%)
Gene names: PFKF, PFKP
Sequence domains: Phosphofructokinase

Ligands and Environments

4 bound ligands:
Ligand PO4 6 x PO4
Ligand FBP 4 x FBP
Ligand ADP 2 x ADP
Ligand F6P 2 x F6P
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P21
Unit cell:
a: 76.652Å b: 164.524Å c: 133.2Å
α: 90° β: 102.96° γ: 90°
R-values:
R R work R free
0.218 0.216 0.255
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Protein Ensembles: How Does Nature Harness Thermodynamic Fluctuations for Life? The Diverse Functional Roles of Conformational Ensembles in the Cell.
Wei et al. (2016)
1 more

3 mentions without citation

Therapeutic Targeting the Allosteric Cysteinome of RAS and Kinase Families.
Li et al. (2022)
2 more