› 4zpz

X-ray diffraction
1.54Å resolution

Crystal Structure of Semi-synthetic Ubiquitin with Phospho-Ser65 and Ala46Cys

Released:
DOI: 10.2210/pdb4zpz/pdb
PDB entry 4zpz coloured by chain, front view.
PDB entry 4zpz coloured by chain, side view.
PDB entry 4zpz coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
A Versatile Strategy for the Semisynthetic Production of Ser65 Phosphorylated Ubiquitin and Its Biochemical and Structural Characterisation.
Han C, Pao KC, Kazlauskaite A, Muqit MM, Virdee S
OpenAccess logo Chembiochem 16 1574-9 (2015)
PMID: 26010437

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-143296 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ubiquitin Chains: A, B
Molecule details ›
Chains: A, B
Length: 76 amino acids
Theoretical weight: 8.69 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P0CG47 (Residues: 153-225; Coverage: 32%)
Gene name: UBB
Sequence domains: Ubiquitin family

Ligands and Environments

No bound ligands
1 modified residue:
Ligand SEP 2 x SEP

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P31
Unit cell:
a: 41.699Å b: 41.699Å c: 69.831Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.14 0.139 0.173
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Expanding the ubiquitin code through post-translational modification.
Herhaus et al. (2015)
2 more

2 mentions without citation

A Versatile Strategy for the Semisynthetic Production of Ser65 Phosphorylated Ubiquitin and Its Biochemical and Structural Characterisation.
Han et al. (2015)
1 more