› 4a3u

X-ray diffraction
1.7Å resolution

X-structure of the old yellow enzyme homologue from zymomonas mobilis (NCR)

Released:
DOI: 10.2210/pdb4a3u/pdb
PDB entry 4a3u coloured by chain, front view.
PDB entry 4a3u coloured by chain, side view.
PDB entry 4a3u coloured by chain, top view.
Source organism: Zymomonas mobilis
Primary publication:
Crystal structure determination and mutagenesis analysis of the ene reductase NCR.
Reich S, Hoeffken HW, Rosche B, Nestl BM, Hauer B
Chembiochem 13 2400-7 (2012)
PMID: 23033175

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177720 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NADH:flavin oxidoreductase/NADH oxidase N-terminal domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 358 amino acids
Theoretical weight: 39.54 KDa
Source organism: Zymomonas mobilis
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5NLA1 (Residues: 1-358; Coverage: 100%)
Gene name: ZMO1885
Sequence domains: NADH:flavin oxidoreductase / NADH oxidase family
Structure domains: Aldolase class I

Ligands and Environments

5 bound ligands:
Ligand FMN 2 x FMN
Ligand ACT 2 x ACT
Ligand NCA 2 x NCA
Ligand NA 3 x NA
Ligand K 2 x K
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P21
Unit cell:
a: 52.6Å b: 90.5Å c: 76.6Å
α: 90° β: 108.3° γ: 90°
R-values:
R R work R free
0.242 0.239 0.303
Expression system: Escherichia coli

Quick links

Citations

2 review citations

New developments in 'ene'-reductase catalysed biological hydrogenations.
Toogood et al. (2014)
1 more

3 mentions without citation

Crystal structure determination and mutagenesis analysis of the ene reductase NCR.
Reich et al. (2012)
2 more