› 4amj

X-ray diffraction
2.3Å resolution

Turkey beta1 adrenergic receptor with stabilising mutations and bound biased agonist carvedilol

Released:
DOI: 10.2210/pdb4amj/pdb
PDB entry 4amj coloured by chain, front view.
PDB entry 4amj coloured by chain, side view.
PDB entry 4amj coloured by chain, top view.
Source organism: Meleagris gallopavo
Primary publication:
Crystal structures of a stabilized β1-adrenoceptor bound to the biased agonists bucindolol and carvedilol.
Warne T, Edwards PC, Leslie AG, Tate CG
OpenAccess logo Structure 20 841-9 (2012)
PMID: 22579251

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139703 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-1 adrenergic receptor Chains: A, B
Molecule details ›
Chains: A, B
Length: 315 amino acids
Theoretical weight: 35.94 KDa
Source organism: Meleagris gallopavo
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P07700 (Residues: 33-368; Coverage: 64%)
Gene name: ADRB1
Sequence domains: 7 transmembrane receptor (rhodopsin family)
Structure domains: Rhodopsin 7-helix transmembrane proteins

Ligands and Environments

3 bound ligands:
Ligand CVD 2 x CVD
Ligand NA 2 x NA
Ligand 2CV 12 x 2CV
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P21
Unit cell:
a: 90.056Å b: 62.196Å c: 100.926Å
α: 90° β: 109.17° γ: 90°
R-values:
R R work R free
0.204 0.202 0.24
Expression system: Trichoplusia ni

Quick links

Citations

48 review citations

Molecular signatures of G-protein-coupled receptors.
Venkatakrishnan et al. (2013)
47 more

31 mentions without citation

Structure-function of the G protein-coupled receptor superfamily.
Katritch et al. (2013)
30 more