› 4chk

X-ray diffraction
2.85Å resolution

Crystal Structure of the ARF5 oligomerization domain

Released:
DOI: 10.2210/pdb4chk/pdb
PDB entry 4chk coloured by chain, front view.
PDB entry 4chk coloured by chain, side view.
PDB entry 4chk coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
Structural basis for oligomerization of auxin transcriptional regulators.
Nanao MH, Vinos-Poyo T, Brunoud G, Thévenon E, Mazzoleni M, Mast D, Lainé S, Wang S, Hagen G, Li H, Guilfoyle TJ, Parcy F, Vernoux T, Dumas R
Nat Commun 5 3617 (2014)
PMID: 24710426

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161063 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Auxin response factor 5 Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 127 amino acids
Theoretical weight: 14.09 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P93024 (Residues: 779-902; Coverage: 14%)
Gene names: ARF5, At1g19850, F6F9.10, IAA24, MP
Sequence domains: AUX/IAA family
Structure domains: Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: C2
Unit cell:
a: 196.264Å b: 86.438Å c: 91.371Å
α: 90° β: 112.71° γ: 90°
R-values:
R R work R free
0.184 0.182 0.22
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

26 review citations

Auxin Signaling.
Leyser O. (2018)
25 more

7 mentions without citation

Refining the nuclear auxin response pathway through structural biology.
Korasick et al. (2015)
6 more