› 4d12

X-ray diffraction
1.4Å resolution

Crystal Structure of Cofactor-free Urate Oxidase Anaerobically Complexed with Uric Acid

Released:
DOI: 10.2210/pdb4d12/pdb
PDB entry 4d12 coloured by chain, front view.
PDB entry 4d12 coloured by chain, side view.
PDB entry 4d12 coloured by chain, top view.
Source organism: Aspergillus flavus
Primary publication:
Direct evidence for a peroxide intermediate and a reactive enzyme-substrate-dioxygen configuration in a cofactor-free oxidase.
Bui S, von Stetten D, Jambrina PG, Prangé T, Colloc'h N, de Sanctis D, Royant A, Rosta E, Steiner RA
OpenAccess logo Angew Chem Int Ed Engl 53 13710-4 (2014)
PMID: 25314114

Function and Biology Details

Reaction catalysed: 
Urate + O(2) + H(2)O = 5-hydroxyisourate + H(2)O(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169432 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Uricase Chain: A
Molecule details ›
Chain: A
Length: 302 amino acids
Theoretical weight: 34.29 KDa
Source organism: Aspergillus flavus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q00511 (Residues: 1-302; Coverage: 100%)
Gene names: Uox, uaZ
Sequence domains: Uricase
Structure domains: Urate Oxidase;

Ligands and Environments

2 bound ligands:
Ligand MPD 1 x MPD
Ligand URC 1 x URC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AGILENT NOVA
Spacegroup: I222
Unit cell:
a: 78.918Å b: 94.87Å c: 104.15Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.119 0.118 0.142
Expression system: Escherichia coli

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Citations

3 review citations

Radiation damage to macromolecules: kill or cure?
Garman et al. (2015)
2 more

8 mentions without citation

Glycotope sharing between snail hemolymph and larval schistosomes: larval transformation products alter shared glycan patterns of plasma proteins.
Yoshino et al. (2012)
7 more