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X-ray diffraction
2.5Å resolution

Crystal structure of Cryptosporidium parvum pyruvate kinase

Released:
DOI: 10.2210/pdb4drs/pdb
PDB entry 4drs coloured by chain, front view.
PDB entry 4drs coloured by chain, side view.
PDB entry 4drs coloured by chain, top view.
Source organism: Cryptosporidium parvum Iowa II
Primary publication:
Crystal structure of Cryptosporidium parvum pyruvate kinase.
Cook WJ, Senkovich O, Aleem K, Chattopadhyay D
OpenAccess logo PLoS One 7 e46875 (2012)
PMID: 23056503

Function and Biology Details

Reaction catalysed: 
ATP + pyruvate = ADP + phosphoenolpyruvate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176930 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pyruvate kinase Chains: A, B
Molecule details ›
Chains: A, B
Length: 526 amino acids
Theoretical weight: 56.49 KDa
Source organism: Cryptosporidium parvum Iowa II
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5CSM7 (Residues: 7-532; Coverage: 99%)
Gene name: cgd1_2040
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand SO4 4 x SO4
Ligand GOL 4 x GOL
Ligand ACT 6 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P21212
Unit cell:
a: 129.895Å b: 136.941Å c: 77.233Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.215 0.213 0.249
Expression system: Escherichia coli

Quick links

Citations

7 citation in other articles

Biochemical and structural characterization of Cryptosporidium parvum Lactate dehydrogenase.
Cook et al. (2015)
6 more

3 mentions without citation

An overview of structure, function, and regulation of pyruvate kinases.
Schormann et al. (2019)
2 more