› 4dva

X-ray diffraction
1.94Å resolution

The crystal structure of human urokinase-type plasminogen activator catalytic domain

Released:
DOI: 10.2210/pdb4dva/pdb
PDB entry 4dva coloured by chain, front view.
PDB entry 4dva coloured by chain, side view.
PDB entry 4dva coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Rezymogenation of active urokinase induced by an inhibitory antibody.
Jiang L, Botkjaer KA, Andersen LM, Yuan C, Andreasen PA, Huang M
Biochem J 449 161-6 (2013)
PMID: 23016918

Function and Biology Details

Reaction catalysed: 
Specific cleavage of Arg-|-Val bond in plasminogen to form plasmin.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133249 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Urokinase-type plasminogen activator chain B Chain: U
Molecule details ›
Chain: U
Length: 246 amino acids
Theoretical weight: 27.72 KDa
Source organism: Homo sapiens
Expression system: Komagataella pastoris
UniProt:
  • Canonical: P00749 (Residues: 179-424; Coverage: 60%)
Gene name: PLAU
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases

Ligands and Environments

2 bound ligands:
Ligand SO4 2 x SO4
Ligand P6G 2 x P6G
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: R3
Unit cell:
a: 121.281Å b: 121.281Å c: 42.806Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.2 0.197 0.244
Expression system: Komagataella pastoris

Quick links

Citations

8 citation in other articles

Development of a 'mouse and human cross-reactive' affinity-matured exosite inhibitory human antibody specific to TACE (ADAM17) for cancer immunotherapy.
Kwok et al. (2014)
7 more

9 mentions without citation

Structural Biology and Protein Engineering of Thrombolytics.
Mican et al. (2019)
8 more