› 4dxg

X-ray diffraction
2.5Å resolution

Crystal structure of Staphylococcal Superantigen-Like protein 4 complexed with sialyl Lewis X

Released:
DOI: 10.2210/pdb4dxg/pdb
PDB entry 4dxg coloured by chain, front view.
PDB entry 4dxg coloured by chain, side view.
PDB entry 4dxg coloured by chain, top view.
Source organism: Staphylococcus aureus subsp. aureus str. Newman
Primary publication:
Structural and functional properties of staphylococcal superantigen-like protein 4.
Hermans SJ, Baker HM, Sequeira RP, Langley RJ, Baker EN, Fraser JD
Infect Immun 80 4004-13 (2012)
PMID: 22949551

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-494130 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Staphylococcal superantigen-like 4 Chain: A
Molecule details ›
Chain: A
Length: 204 amino acids
Theoretical weight: 23.4 KDa
Source organism: Staphylococcus aureus subsp. aureus str. Newman
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0H3K6A3 (Residues: 109-308; Coverage: 72%)
Gene names: NWMN_0391, ssl4
Sequence domains:
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NDG, GAL, SIA, FUC
Carbohydrate polymer
2 bound ligands:
Ligand PIN 1 x PIN
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: I222
Unit cell:
a: 61.88Å b: 78.88Å c: 97.701Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.188 0.185 0.254
Expression system: Escherichia coli

Quick links

Citations

8 review citations

Staphylococcal manipulation of host immune responses.
Thammavongsa et al. (2015)
7 more

3 mentions without citation

Structural basis for inhibition of TLR2 by staphylococcal superantigen-like protein 3 (SSL3).
Koymans et al. (2015)
2 more