› 4ebk

X-ray diffraction
2.15Å resolution

Crystal structure of aminoglycoside 4'-O-adenylyltransferase ANT(4')-IIb, tobramycin-bound

Released:
DOI: 10.2210/pdb4ebk/pdb
PDB entry 4ebk coloured by chain, front view.
PDB entry 4ebk coloured by chain, side view.
PDB entry 4ebk coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Entry authors: Stogios PJ, Dong A, Minasov G, Evdokimova E, Egorova O, Yim V, Kudritska M, Courvalin P, Savchenko A, Anderson WF, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-111953 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aminoglycoside nucleotidyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 272 amino acids
Theoretical weight: 30.82 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: D0E7M2 (Residues: 2-251; Coverage: 100%)
Gene name: ant(4')-IIb
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand TOY 2 x TOY
Ligand EDO 2 x EDO
Ligand IOD 12 x IOD
Ligand CL 17 x CL
2 modified residues:
Ligand MSE 10 x MSE
Ligand IYR 2 x IYR

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P212121
Unit cell:
a: 81.906Å b: 84.451Å c: 98.762Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.174 0.172 0.221
Expression system: Escherichia coli BL21(DE3)

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