› 4eg2

X-ray diffraction
2.2Å resolution

2.2 Angstrom Crystal Structure of Cytidine deaminase from Vibrio cholerae in Complex with Zinc and Uridine

Released:
DOI: 10.2210/pdb4eg2/pdb
PDB entry 4eg2 coloured by chain, front view.
PDB entry 4eg2 coloured by chain, side view.
PDB entry 4eg2 coloured by chain, top view.
Source organism: Vibrio cholerae
Entry authors: Minasov G, Wawrzak Z, Skarina T, Wang Y, Grimshaw S, Papazisi L, Savchenko A, Anderson WF, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Reaction catalysed: 
2'-deoxycytidine + H(2)O = 2'-deoxyuridine + NH(3)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192071 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytidine deaminase Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 298 amino acids
Theoretical weight: 32.77 KDa
Source organism: Vibrio cholerae
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: Q9KSM5 (Residues: 1-295; Coverage: 100%)
Gene names: VC_1231, cdd
Sequence domains:
Structure domains: Cytidine Deaminase, domain 2

Ligands and Environments

4 bound ligands:
Ligand ZN 8 x ZN
Ligand URI 8 x URI
Ligand ACT 9 x ACT
Ligand MG 1 x MG
1 modified residue:
Ligand MSE 88 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: P21
Unit cell:
a: 68.81Å b: 163.73Å c: 111.95Å
α: 90° β: 97.7° γ: 90°
R-values:
R R work R free
0.162 0.159 0.21
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

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