› 4evx

X-ray diffraction
1.7Å resolution

Crystal structure of putative phage endolysin from S. enterica

Released:
DOI: 10.2210/pdb4evx/pdb
PDB entry 4evx coloured by chain, front view.
PDB entry 4evx coloured by chain, side view.
PDB entry 4evx coloured by chain, top view.
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
Entry authors: Michalska K, Li H, Jedrzejczak R, Brown RN, Cort JR, Heffron F, Nakayasu ES, Adkins JN, Joachimiak A, Program for the Characterization of Secreted Effector Proteins (PCSEP), Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assemblies composition:
homo tetramer
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-187257 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Lysozyme Chains: A, B
Molecule details ›
Chains: A, B
Length: 106 amino acids
Theoretical weight: 12.26 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8ZLC6 (Residues: 5-107; Coverage: 87%)
Gene name: STM3605
Sequence domains: Phage lysozyme
Structure domains: Rna Polymerase Sigma Factor; Chain: A

Ligands and Environments

No bound ligands
3 modified residues:
Ligand CSO 2 x CSO
Ligand MLY 4 x MLY
Ligand MSE 4 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P6122
Unit cell:
a: 54.4Å b: 54.4Å c: 261.09Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.196 0.195 0.215
Expression system: Escherichia coli BL21(DE3)

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