› 4fab

X-ray diffraction
2.7Å resolution

THREE-DIMENSIONAL STRUCTURE OF A FLUORESCEIN-FAB COMPLEX CRYSTALLIZED IN 2-METHYL-2,4-PENTANEDIOL

Released:
DOI: 10.2210/pdb4fab/pdb
PDB entry 4fab coloured by chain, front view.
PDB entry 4fab coloured by chain, side view.
PDB entry 4fab coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Three-dimensional structure of a fluorescein-Fab complex crystallized in 2-methyl-2,4-pentanediol.
Herron JN, He XM, Mason ML, Voss EW, Edmundson AB
Proteins 5 271-80 (1989)
PMID: 2508085

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-208656 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
IGG2A-KAPPA 4-4-20 FAB (LIGHT CHAIN) Chain: L
Molecule details ›
Chain: L
Length: 219 amino acids
Theoretical weight: 24.19 KDa
Source organism: Mus musculus
Expression system: Not provided
Structure domains: Immunoglobulins
Immunoglobulin heavy constant gamma 2A Chain: H
Molecule details ›
Chain: H
Length: 216 amino acids
Theoretical weight: 23.66 KDa
Source organism: Mus musculus
Expression system: Not provided
UniProt:
  • Canonical: P01865 (Residues: 1-97; Coverage: 24%)
Gene names: Igh-1a, Ighg2a
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins

Ligands and Environments

2 bound ligands:
Ligand FLU 1 x FLU
Ligand MPD 1 x MPD
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P1
Unit cell:
a: 58.3Å b: 43.9Å c: 42.5Å
α: 82.1° β: 87.3° γ: 84.6°
R-values:
R R work R free
0.215 not available not available
Expression system: Not provided

Quick links

Citations

19 review citations

Conformations of immunoglobulin hypervariable regions.
Chothia et al. (1989)
18 more

11 mentions without citation

Immunotoxins constructed with ribosome-inactivating proteins and their enhancers: a lethal cocktail with tumor specific efficacy.
Gilabert-Oriol et al. (2014)
10 more