› 4ffi

X-ray diffraction
2.3Å resolution

Crystal Structure of Levan Fructotransferase D54N mutant from Arthrobacter ureafaciens in complex with levanbiose

Released:
DOI: 10.2210/pdb4ffi/pdb
PDB entry 4ffi coloured by chain, front view.
PDB entry 4ffi coloured by chain, side view.
PDB entry 4ffi coloured by chain, top view.
Source organism: Paenarthrobacter ureafaciens
Primary publication:
Structural and functional basis for substrate specificity and catalysis of levan fructotransferase.
Park J, Kim MI, Park YD, Shin I, Cha J, Kim CH, Rhee S
J Biol Chem 287 31233-41 (2012)
PMID: 22810228

Function and Biology Details

Reaction catalysed: 
Produces di-beta-D-fructofuranose 2,6':2',6-dianhydride (DFA IV) by successively eliminating the diminishing (2->6)-beta-D-fructan (levan) chain from the terminal D-fructosyl-D-fructosyl disaccharide.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191903 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (3 distinct):
Levan fructotransferase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 492 amino acids
Theoretical weight: 54.39 KDa
Source organism: Paenarthrobacter ureafaciens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9KJD0 (Residues: 41-521; Coverage: 92%)
Gene name: lftA
Sequence domains:
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: FRU
Carbohydrate polymer
Carbohydrate polymer : NEW Components: FRU
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-17A
Spacegroup: P212121
Unit cell:
a: 82.511Å b: 168.125Å c: 263.379Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.215 0.215 0.251
Expression system: Escherichia coli

Quick links

Citations

1 review citation

From fructans to difructose dianhydrides.
Wang et al. (2015)
11 other articles

3 mentions without citation

Structural and functional basis for substrate specificity and catalysis of levan fructotransferase.
Park et al. (2012)
2 more