› 4fxj

X-ray diffraction
2.9Å resolution

Structure of M2 pyruvate kinase in complex with phenylalanine

Released:
DOI: 10.2210/pdb4fxj/pdb
PDB entry 4fxj coloured by chain, front view.
PDB entry 4fxj coloured by chain, side view.
PDB entry 4fxj coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
M2 pyruvate kinase provides a mechanism for nutrient sensing and regulation of cell proliferation.
Morgan HP, O'Reilly FJ, Wear MA, O'Neill JR, Fothergill-Gilmore LA, Hupp T, Walkinshaw MD
Proc Natl Acad Sci U S A 110 5881-6 (2013)
PMID: 23530218

Function and Biology Details

Reactions catalysed: 
ATP + pyruvate = ADP + phosphoenolpyruvate
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146985 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pyruvate kinase PKM Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 551 amino acids
Theoretical weight: 60.16 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P14618 (Residues: 1-531; Coverage: 100%)
Gene names: OIP3, PK2, PK3, PKM, PKM2
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand PO4 5 x PO4
Ligand PHE 4 x PHE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: P21
Unit cell:
a: 97.26Å b: 70.6Å c: 167.6Å
α: 90° β: 105.72° γ: 90°
R-values:
R R work R free
0.248 0.246 0.297
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

21 review citations

The return of metabolism: biochemistry and physiology of the pentose phosphate pathway.
Stincone et al. (2015)
20 more

11 mentions without citation

Tumor pyruvate kinase M2 modulators: a comprehensive account of activators and inhibitors as anticancer agents.
Rathod et al. (2021)
10 more