› 4gll

X-ray diffraction
2.5Å resolution

Crystal structure of human UDP-xylose synthase.

Released:
DOI: 10.2210/pdb4gll/pdb
PDB entry 4gll coloured by chain, front view.
PDB entry 4gll coloured by chain, side view.
PDB entry 4gll coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Human UDP-α-D-xylose synthase and Escherichia coli ArnA conserve a conformational shunt that controls whether xylose or 4-keto-xylose is produced.
Polizzi SJ, Walsh RM, Peeples WB, Lim JM, Wells L, Wood ZA
Biochemistry 51 8844-55 (2012)
PMID: 23072385

Function and Biology Details

Reaction catalysed: 
UDP-D-glucuronate = UDP-D-xylose + CO(2)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-185481 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
UDP-glucuronic acid decarboxylase 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 337 amino acids
Theoretical weight: 38.06 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8NBZ7 (Residues: 85-420; Coverage: 80%)
Gene names: UNQ2538/PRO6079, UXS1
Sequence domains: GDP-mannose 4,6 dehydratase
Structure domains:

Ligands and Environments


Cofactor: Ligand NAD 2 x NAD
2 bound ligands:
Ligand GAI 2 x GAI
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: P3221
Unit cell:
a: 125.61Å b: 125.61Å c: 98.92Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.209 0.178 0.223
Expression system: Escherichia coli

Quick links

Citations

13 citation in other articles

Crystal structure of the capsular polysaccharide synthesizing protein CapE of Staphylococcus aureus.
Miyafusa et al. (2013)
12 more

1 mention without citation

Human UDP-α-D-xylose synthase and Escherichia coli ArnA conserve a conformational shunt that controls whether xylose or 4-keto-xylose is produced.
Polizzi et al. (2012)