› 4hhs

X-ray diffraction
1.7Å resolution

Crystal Structure of fatty acid alpha-dioxygenase (Arabidopsis thaliana)

Released:
DOI: 10.2210/pdb4hhs/pdb
PDB entry 4hhs coloured by chain, front view.
PDB entry 4hhs coloured by chain, side view.
PDB entry 4hhs coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
The crystal structure of α-Dioxygenase provides insight into diversity in the cyclooxygenase-peroxidase superfamily.
Goulah CC, Zhu G, Koszelak-Rosenblum M, Malkowski MG
Biochemistry 52 1364-72 (2013)
PMID: 23373518

Function and Biology Details

Reaction catalysed: 
A fatty acid + O(2) = a (2R)-2-hydroperoxyfatty acid
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193456 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alpha-dioxygenase 1 Chain: A
Molecule details ›
Chain: A
Length: 652 amino acids
Theoretical weight: 74.82 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9SGH6 (Residues: 1-639; Coverage: 100%)
Gene names: At3g01420, DIOX1, DOX1, PADOX-1, PIOX, T13O15.6
Sequence domains: Animal haem peroxidase
Structure domains: Haem peroxidase domain superfamily, animal type

Ligands and Environments

7 bound ligands:
Ligand HEM 1 x HEM
Ligand CA 5 x CA
Ligand CL 3 x CL
Ligand PEG 1 x PEG
Ligand BNG 1 x BNG
Ligand P6G 1 x P6G
Ligand PGE 1 x PGE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: P4322
Unit cell:
a: 102.15Å b: 102.15Å c: 137.565Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.161 0.159 0.187
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Production of cross-kingdom oxylipins by pathogenic fungi: An update on their role in development and pathogenicity.
Fischer et al. (2016)
2 more

3 mentions without citation

Monotopic Membrane Proteins Join the Fold.
Allen et al. (2019)
2 more