› 4hph

X-ray diffraction
1.7Å resolution

The crystal structure of isomaltulose synthase mutant E295Q from Erwinia rhapontici NX5 in complex with its natural substrate sucrose

Released:
DOI: 10.2210/pdb4hph/pdb
PDB entry 4hph coloured by chain, front view.
PDB entry 4hph coloured by chain, side view.
PDB entry 4hph coloured by chain, top view.
Source organism: Erwinia rhapontici
Primary publication:
The structural basis of Erwinia rhapontici isomaltulose synthase.
Xu Z, Li S, Li J, Li Y, Feng X, Wang R, Xu H, Zhou J
OpenAccess logo PLoS One 8 e74788 (2013)
PMID: 24069347

Function and Biology Details

Reaction catalysed: 
Sucrose = 6-O-alpha-D-glucopyranosyl-D-fructofuranose
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-112985 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Glycosyl hydrolase family 13 catalytic domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 559 amino acids
Theoretical weight: 65.83 KDa
Source organism: Erwinia rhapontici
Expression system: Escherichia coli
UniProt:
  • Canonical: D9MPF2 (Residues: 42-600; Coverage: 93%)
Sequence domains:
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC, FRU
Carbohydrate polymer
2 bound ligands:
Ligand CA 1 x CA
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P212121
Unit cell:
a: 58.553Å b: 81.091Å c: 138.354Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.163 0.161 0.2
Expression system: Escherichia coli

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Citations

2 review citations

Biotechnical production of trehalose through the trehalose synthase pathway: current status and future prospects.
Cai et al. (2018)
1 more

4 mentions without citation

Insight into the substrate specificity change caused by the Y227H mutation of α-glucosidase III from the European honeybee (Apis mellifera) through molecular dynamics simulations.
Na Ayutthaya et al. (2018)
3 more