› 4i5h

X-ray diffraction
1.9Å resolution

Crystal Structure of a Double Mutant Rat Erk2 Complexed With a Type II Quinazoline Inhibitor

Released:
DOI: 10.2210/pdb4i5h/pdb
PDB entry 4i5h coloured by chain, front view.
PDB entry 4i5h coloured by chain, side view.
PDB entry 4i5h coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Sequence determinants of a specific inactive protein kinase conformation.
Hari SB, Merritt EA, Maly DJ
Chem Biol 20 806-15 (2013)
PMID: 23790491

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158941 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Mitogen-activated protein kinase 1 Chain: A
Molecule details ›
Chain: A
Length: 359 amino acids
Theoretical weight: 41.35 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P63086 (Residues: 2-358; Coverage: 100%)
Gene names: Erk2, Mapk, Mapk1, Prkm1
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand G17 1 x G17
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P3121
Unit cell:
a: 77.24Å b: 77.24Å c: 121.791Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.22 0.218 0.256
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

16 review citations

Targeting cancer with kinase inhibitors.
Gross et al. (2015)
15 more

2 mentions without citation

Sequence determinants of a specific inactive protein kinase conformation.
Hari et al. (2013)
1 more