› 4jfr

X-ray diffraction
2.17Å resolution

Crystal structure of anabolic ornithine carbamoyltransferase from Vibrio vulnificus in complex with carbamoyl phosphate

Released:
DOI: 10.2210/pdb4jfr/pdb
PDB entry 4jfr coloured by chain, front view.
PDB entry 4jfr coloured by chain, side view.
PDB entry 4jfr coloured by chain, top view.
Source organism: Vibrio vulnificus CMCP6
Entry authors: Shabalin IG, Winsor J, Grimshaw S, Osinski T, Bajor J, Chordia MD, Shuvalova L, Anderson WF, Minor W, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Reaction catalysed: 
Carbamoyl phosphate + L-ornithine = phosphate + L-citrulline
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-184043 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ornithine carbamoyltransferase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 358 amino acids
Theoretical weight: 39.64 KDa
Source organism: Vibrio vulnificus CMCP6
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q8DCF5 (Residues: 1-334; Coverage: 100%)
Gene names: VV1_1466, argF
Sequence domains:
Structure domains: Aspartate/ornithine carbamoyltransferase

Ligands and Environments

3 bound ligands:
Ligand CP 2 x CP
Ligand CL 3 x CL
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: P212121
Unit cell:
a: 81.343Å b: 80.916Å c: 177.637Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.173 0.171 0.205
Expression system: Escherichia coli BL21

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