› 4jz8

X-ray diffraction
2.1Å resolution

Carbamate kinase from Giardia lamblia bound to citric acid

Released:
DOI: 10.2210/pdb4jz8/pdb
PDB entry 4jz8 coloured by chain, front view.
PDB entry 4jz8 coloured by chain, side view.
PDB entry 4jz8 coloured by chain, top view.
Source organism: Giardia lamblia ATCC 50803
Primary publication:
Crystal structures of carbamate kinase from Giardia lamblia bound with citric acid and AMP-PNP.
Lim K, Kulakova L, Galkin A, Herzberg O
OpenAccess logo PLoS One 8 e64004 (2013)
PMID: 23700444

Function and Biology Details

Reaction catalysed: 
(1a) ATP + carbamate = ADP + carbamoyl phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-108579 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Carbamate kinase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 317 amino acids
Theoretical weight: 33.98 KDa
Source organism: Giardia lamblia ATCC 50803
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A8BB85 (Residues: 1-316; Coverage: 100%)
Gene name: GL50803_0016453
Sequence domains: Amino acid kinase family
Structure domains: Acetylglutamate kinase-like

Ligands and Environments

1 bound ligand:
Ligand CIT 4 x CIT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: P21
Unit cell:
a: 69.928Å b: 86.401Å c: 101.96Å
α: 90° β: 106.53° γ: 90°
R-values:
R R work R free
0.218 0.218 0.286
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Parasitic diarrheal disease: drug development and targets.
Azam et al. (2015)
1 other article

1 mention without citation

Crystal structures of carbamate kinase from Giardia lamblia bound with citric acid and AMP-PNP.
Lim et al. (2013)