› 4jzb

X-ray diffraction
1.9Å resolution

Crystal Structure of Leshmaniasis major Farnesyl diphosphate synthase in complex with 1-(2-HYDROXY-2,2-DIPHOSPHONOETHYL)-3-PHENYLPYRIDINIUM, IPP and Ca2+

Released:
DOI: 10.2210/pdb4jzb/pdb
PDB entry 4jzb coloured by chain, front view.
PDB entry 4jzb coloured by chain, side view.
PDB entry 4jzb coloured by chain, top view.
Source organism: Leishmania major
Primary publication:
Structural and thermodynamic basis of the inhibition of Leishmania major farnesyl diphosphate synthase by nitrogen-containing bisphosphonates.
Aripirala S, Gonzalez-Pacanowska D, Oldfield E, Kaiser M, Amzel LM, Gabelli SB
Acta Crystallogr D Biol Crystallogr 70 802-10 (2014)
PMID: 24598749

Function and Biology Details

Reactions catalysed: 
Prenyl diphosphate + isopentenyl diphosphate = diphosphate + geranyl diphosphate
Geranyl diphosphate + isopentenyl diphosphate = diphosphate + (2E,6E)-farnesyl diphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175690 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Farnesyl pyrophosphate synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 362 amino acids
Theoretical weight: 41.05 KDa
Source organism: Leishmania major
Expression system: Escherichia coli
UniProt:
  • Canonical: Q4QBL1 (Residues: 2-362; Coverage: 100%)
Gene names: FPPS, LMJF_22_1360, geranyltranstransferase
Sequence domains: Polyprenyl synthetase
Structure domains: Farnesyl Diphosphate Synthase

Ligands and Environments

4 bound ligands:
Ligand CA 6 x CA
Ligand IPE 2 x IPE
Ligand P2H 2 x P2H
Ligand NA 2 x NA
1 modified residue:
Ligand FME 2 x FME

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E DW
Spacegroup: P212121
Unit cell:
a: 80.361Å b: 85.967Å c: 107.074Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.175 0.173 0.21
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Carbonic anhydrases from Trypanosoma and Leishmania as anti-protozoan drug targets.
Vermelho et al. (2017)
3 more

6 mentions without citation

Phosphonate and Bisphosphonate Inhibitors of Farnesyl Pyrophosphate Synthases: A Structure-Guided Perspective.
Park et al. (2020)
5 more