› 4k28

X-ray diffraction
2.15Å resolution

2.15 Angstrom resolution crystal structure of a shikimate dehydrogenase family protein from Pseudomonas putida KT2440 in complex with NAD+

Released:
DOI: 10.2210/pdb4k28/pdb
PDB entry 4k28 coloured by chain, front view.
PDB entry 4k28 coloured by chain, side view.
PDB entry 4k28 coloured by chain, top view.
Source organism: Pseudomonas putida KT2440
Primary publication:
Insights into the function of RifI2: structural and biochemical investigation of a new shikimate dehydrogenase family protein.
Peek J, Garcia C, Lee J, Christendat D
Biochim Biophys Acta 1834 516-23 (2013)
PMID: 23142411

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-183480 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Shikimate dehydrogenase family protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 269 amino acids
Theoretical weight: 28.1 KDa
Source organism: Pseudomonas putida KT2440
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q88JP1 (Residues: 1-269; Coverage: 100%)
Gene name: PP_2608
Sequence domains: Shikimate dehydrogenase substrate binding domain
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand NAD 2 x NAD
Ligand MN 6 x MN
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: P212121
Unit cell:
a: 44.437Å b: 76.688Å c: 153.333Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.182 0.179 0.238
Expression system: Escherichia coli BL21

Quick links

Citations

1 review citation

Mycobacterium tuberculosis Shikimate Pathway Enzymes as Targets for the Rational Design of Anti-Tuberculosis Drugs.
Nunes et al. (2020)
1 other article