› 4kkl

X-ray diffraction
2.85Å resolution

Structure of the E148A mutant of CLC-ec1 delta NC construct in 100mM fluoride

Released:
DOI: 10.2210/pdb4kkl/pdb
PDB entry 4kkl coloured by chain, front view.
PDB entry 4kkl coloured by chain, side view.
PDB entry 4kkl coloured by chain, top view.
Source organisms:
Primary publication:
Fluoride-dependent interruption of the transport cycle of a CLC Cl-/H+ antiporter.
Lim HH, Stockbridge RB, Miller C
Nat Chem Biol 9 721-5 (2013)
PMID: 24036509

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
hetero dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-153303 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
H(+)/Cl(-) exchange transporter ClcA Chains: A, B
Molecule details ›
Chains: A, B
Length: 446 amino acids
Theoretical weight: 47.56 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P37019 (Residues: 16-460; Coverage: 94%)
Gene names: JW5012, b0155, clcA, eriC, yadQ
Sequence domains: Voltage gated chloride channel
Structure domains: Clc chloride channel
Fab, heavy chain Chains: C, E
Molecule details ›
Chains: C, E
Length: 222 amino acids
Theoretical weight: 23.82 KDa
Source organism: Mus musculus
Expression system: Mus musculus
Structure domains: Immunoglobulins
Fab, light chain Chains: D, F
Molecule details ›
Chains: D, F
Length: 211 amino acids
Theoretical weight: 23.09 KDa
Source organism: Mus musculus
Expression system: Mus musculus
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
Ligand F 4 x F
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: C2
Unit cell:
a: 231.42Å b: 100.094Å c: 170.226Å
α: 90° β: 131.82° γ: 90°
R-values:
R R work R free
0.251 0.248 0.29
Expression systems:
  • Escherichia coli
  • Mus musculus

Quick links

Citations

3 review citations

DENTAL ENAMEL FORMATION AND IMPLICATIONS FOR ORAL HEALTH AND DISEASE.
Lacruz et al. (2017)
2 more