› 4lo4

X-ray diffraction
2.87Å resolution

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-185139 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Hemagglutinin component HA-70 C-terminal domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 190 amino acids
Theoretical weight: 21.94 KDa
Source organism: Clostridium botulinum
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8KHU9 (Residues: 2-189; Coverage: 30%)
Gene name: ha70
Sequence domains: Clostridium enterotoxin
Structure domains: Proaerolysin; Chain A, domain 3
Hemagglutinin component HA-70 C-terminal domain-containing protein Chain: B
Molecule details ›
Chain: B
Length: 433 amino acids
Theoretical weight: 49.15 KDa
Source organism: Clostridium botulinum
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8KHU9 (Residues: 206-626; Coverage: 67%)
Gene name: ha70
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: I4132
Unit cell:
a: 261.75Å b: 261.75Å c: 261.75Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.187 0.186 0.206
Expression system: Escherichia coli

Quick links

Citations

17 review citations

Botulinum neurotoxins: genetic, structural and mechanistic insights.
Rossetto et al. (2014)
16 more

3 mentions without citation

Botulinum and Tetanus Neurotoxins.
Dong et al. (2019)
2 more