› 4mb3

X-ray diffraction
1.55Å resolution

Crystal structure of E153Q mutant of cold-adapted chitinase from Moritella marina

Released:
DOI: 10.2210/pdb4mb3/pdb
PDB entry 4mb3 coloured by chain, front view.
PDB entry 4mb3 coloured by chain, side view.
PDB entry 4mb3 coloured by chain, top view.
Source organism: Moritella marina
Primary publication:
Crystal structures of substrate-bound chitinase from the psychrophilic bacterium Moritella marina and its structure in solution.
Malecki PH, Vorgias CE, Petoukhov MV, Svergun DI, Rypniewski W
Acta Crystallogr D Biol Crystallogr 70 676-84 (2014)
PMID: 24598737

Function and Biology Details

Reaction catalysed: 
Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-109414 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
GH18 domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 528 amino acids
Theoretical weight: 58.62 KDa
Source organism: Moritella marina
Expression system: Escherichia coli
UniProt:
  • Canonical: B1VBB0 (Residues: 23-550; Coverage: 100%)
Gene name: chi60
Sequence domains:
Structure domains:

Ligands and Environments

5 bound ligands:
Ligand NA 4 x NA
Ligand GLY 1 x GLY
Ligand MPD 1 x MPD
Ligand IMD 1 x IMD
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.2
Spacegroup: P3112
Unit cell:
a: 67.657Å b: 67.657Å c: 255.643Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.143 0.141 0.184
Expression system: Escherichia coli

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Citations

1 review citation

Oxygen Activation by Cu LPMOs in Recalcitrant Carbohydrate Polysaccharide Conversion to Monomer Sugars.
Meier et al. (2018)
5 other articles

3 mentions without citation

Discovery, Molecular Mechanisms, and Industrial Applications of Cold-Active Enzymes.
Santiago et al. (2016)
2 more