› 4mu8

X-ray diffraction
1.45Å resolution

Crystal structure of an oxidized form of yeast iso-1-cytochrome c at pH 8.8

Released:
DOI: 10.2210/pdb4mu8/pdb
PDB entry 4mu8 coloured by chain, front view.
PDB entry 4mu8 coloured by chain, side view.
PDB entry 4mu8 coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
Structure of a mitochondrial cytochrome c conformer competent for peroxidase activity.
McClelland LJ, Mou TC, Jeakins-Cooley ME, Sprang SR, Bowler BE
Proc Natl Acad Sci U S A 111 6648-53 (2014)
PMID: 24760830

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132226 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytochrome c isoform 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 108 amino acids
Theoretical weight: 12 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P00044 (Residues: 2-109; Coverage: 99%)
Gene names: CYC1, J1653, YJR048W
Sequence domains: Cytochrome c
Structure domains: Cytochrome c-like domain

Ligands and Environments

4 bound ligands:
Ligand HEC 2 x HEC
Ligand SO4 8 x SO4
Ligand TBU 3 x TBU
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: P6322
Unit cell:
a: 93.621Å b: 93.621Å c: 155.014Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.146 0.145 0.154
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

8 review citations

Known unknowns of cardiolipin signaling: The best is yet to come.
Maguire et al. (2017)
7 more

7 mentions without citation

Relating the multi-functionality of cytochrome c to membrane binding and structural conversion.
Schweitzer-Stenner R. (2018)
6 more