› 4olc

X-ray diffraction
2.6Å resolution

Carbamate kinase from Giardia lamblia thiocarbamoylated by disulfiram on Cys242

Released:
DOI: 10.2210/pdb4olc/pdb
PDB entry 4olc coloured by chain, front view.
PDB entry 4olc coloured by chain, side view.
PDB entry 4olc coloured by chain, top view.
Source organism: Giardia lamblia ATCC 50803
Primary publication:
Structural basis for inactivation of Giardia lamblia carbamate kinase by disulfiram.
Galkin A, Kulakova L, Lim K, Chen CZ, Zheng W, Turko IV, Herzberg O
J Biol Chem 289 10502-10509 (2014)
PMID: 24558036

Function and Biology Details

Reaction catalysed: 
(1a) ATP + carbamate = ADP + carbamoyl phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-108579 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Carbamate kinase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 317 amino acids
Theoretical weight: 33.98 KDa
Source organism: Giardia lamblia ATCC 50803
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A8BB85 (Residues: 1-316; Coverage: 100%)
Gene names: GL50803_0016453, GL50803_16453
Sequence domains: Amino acid kinase family
Structure domains: Acetylglutamate kinase-like

Ligands and Environments

2 bound ligands:
Ligand CIT 4 x CIT
Ligand DCD 3 x DCD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P21
Unit cell:
a: 70.297Å b: 98.178Å c: 102.674Å
α: 90° β: 107.57° γ: 90°
R-values:
R R work R free
0.206 0.206 0.274
Expression system: Escherichia coli BL21(DE3)

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Citations

9 review citations

Recent discovery and development of inhibitors targeting coronaviruses.
Pillaiyar et al. (2020)
8 more

2 mentions without citation

Structural basis for inactivation of Giardia lamblia carbamate kinase by disulfiram.
Galkin et al. (2014)
1 more