› 4pa6

X-ray diffraction
3.36Å resolution

Structure of NavMS pore and C-terminal domain crystallised in the presence of channel blocking compound

Released:
DOI: 10.2210/pdb4pa6/pdb
PDB entry 4pa6 coloured by chain, front view.
PDB entry 4pa6 coloured by chain, side view.
PDB entry 4pa6 coloured by chain, top view.
Source organism: Magnetococcus marinus MC-1
Primary publication:
Prokaryotic NavMs channel as a structural and functional model for eukaryotic sodium channel antagonism.
Bagnéris C, DeCaen PG, Naylor CE, Pryde DC, Nobeli I, Clapham DE, Wallace BA
Proc Natl Acad Sci U S A 111 8428-33 (2014)
PMID: 24850863

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-106115 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ion transport domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 149 amino acids
Theoretical weight: 16.84 KDa
Source organism: Magnetococcus marinus MC-1
Expression system: Escherichia coli
UniProt:
  • Canonical: A0L5S6 (Residues: 130-274; Coverage: 53%)
Gene name: Mmc1_0798
Sequence domains:
Structure domains: Helix Hairpins

Ligands and Environments

3 bound ligands:
Ligand 2CV 4 x 2CV
Ligand 12P 1 x 12P
Ligand NA 4 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: C2221
Unit cell:
a: 79.934Å b: 324.571Å c: 79.766Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.304 0.302 0.342
Expression system: Escherichia coli

Quick links

Citations

15 review citations

The hitchhiker's guide to the voltage-gated sodium channel galaxy.
Ahern et al. (2016)
14 more

2 mentions without citation

Prokaryotic NavMs channel as a structural and functional model for eukaryotic sodium channel antagonism.
Bagnéris et al. (2014)
1 more