› 4pdr

X-ray diffraction
1.85Å resolution

Crystal Structure of a K+ selective NaK mutant in Barium and Sodium

Released:
DOI: 10.2210/pdb4pdr/pdb
PDB entry 4pdr coloured by chain, front view.
PDB entry 4pdr coloured by chain, side view.
PDB entry 4pdr coloured by chain, top view.
Source organism: Bacillus cereus ATCC 14579
Entry authors: Lam Y, Zeng W, Sauer DB, Jiang Y

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182541 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Potassium channel domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 97 amino acids
Theoretical weight: 10.77 KDa
Source organism: Bacillus cereus ATCC 14579
Expression system: Escherichia coli
UniProt:
  • Canonical: Q81HW2 (Residues: 20-110; Coverage: 80%)
Gene name: BC_0669
Sequence domains: Ion channel
Structure domains: Helix Hairpins

Ligands and Environments

3 bound ligands:
Ligand BA 6 x BA
Ligand NA 2 x NA
Ligand MPD 4 x MPD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: I4
Unit cell:
a: 68.042Å b: 68.042Å c: 89.627Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.201 0.2 0.227
Expression system: Escherichia coli

Quick links

Citations

1 mention without citation

The conserved potassium channel filter can have distinct ion binding profiles: structural analysis of rubidium, cesium, and barium binding in NaK2K.
Lam et al. (2014)