› 4ph7

X-ray diffraction
2.55Å resolution

Structure of Osh6p in complex with phosphatidylinositol 4-phosphate

Released:
DOI: 10.2210/pdb4ph7/pdb
PDB entry 4ph7 coloured by chain, front view.
PDB entry 4ph7 coloured by chain, side view.
PDB entry 4ph7 coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
INTRACELLULAR TRANSPORT. Phosphatidylserine transport by ORP/Osh proteins is driven by phosphatidylinositol 4-phosphate.
Moser von Filseck J, Čopič A, Delfosse V, Vanni S, Jackson CL, Bourguet W, Drin G
Science 349 432-6 (2015)
PMID: 26206936

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169684 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Oxysterol-binding protein homolog 6 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 450 amino acids
Theoretical weight: 51.81 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q02201 (Residues: 1-448; Coverage: 100%)
Gene names: OSH6, YK102, YKR003W
Sequence domains: Oxysterol-binding protein

Ligands and Environments

1 bound ligand:
Ligand 2Y5 4 x 2Y5
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P212121
Unit cell:
a: 114.11Å b: 122.26Å c: 141.49Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.19 0.188 0.238
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

80 review citations

Understanding the diversity of membrane lipid composition.
Harayama et al. (2018)
79 more

5 mentions without citation

Mechanisms of Non-Vesicular Exchange of Lipids at Membrane Contact Sites: Of Shuttles, Tunnels and, Funnels.
Egea PF. (2021)
4 more