› 4q0v

X-ray diffraction
1.98Å resolution

Crystal structure of Acinetobacter sp. DL28 L-ribose isomerase mutant E204Q in complex with L-ribulose

Released:
DOI: 10.2210/pdb4q0v/pdb
PDB entry 4q0v coloured by chain, front view.
PDB entry 4q0v coloured by chain, side view.
PDB entry 4q0v coloured by chain, top view.
Source organism: Acinetobacter sp. DL-28
Primary publication:
X-ray structure of a novel L-ribose isomerase acting on a non-natural sugar L-ribose as its ideal substrate.
Yoshida H, Yoshihara A, Teraoka M, Terami Y, Takata G, Izumori K, Kamitori S
FEBS J 281 3150-64 (2014)
PMID: 24846739

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188137 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
L-Ribose isomerase Chain: A
Molecule details ›
Chain: A
Length: 260 amino acids
Theoretical weight: 28.75 KDa
Source organism: Acinetobacter sp. DL-28
Expression system: Escherichia coli
UniProt:
  • Canonical: Q93UQ5 (Residues: 3-249; Coverage: 99%)
Gene name: L-RI
Structure domains: Jelly Rolls

Ligands and Environments

4 bound ligands:
Ligand CO 1 x CO
Ligand QDK 2 x QDK
Ligand RUU 5 x RUU
Ligand NCO 1 x NCO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: F222
Unit cell:
a: 95.85Å b: 107.35Å c: 118.18Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.207 0.203 0.243
Expression system: Escherichia coli

Quick links

Citations

1 review citation

L-Ribose isomerase and mannose-6-phosphate isomerase: properties and applications for L-ribose production.
Xu et al. (2016)
5 other articles

1 mention without citation

X-ray structure of a novel L-ribose isomerase acting on a non-natural sugar L-ribose as its ideal substrate.
Yoshida et al. (2014)