› 4rnu

X-ray diffraction
2.68Å resolution

G303 Circular Permutation of Old Yellow Enzyme

Released:
DOI: 10.2210/pdb4rnu/pdb
PDB entry 4rnu coloured by chain, front view.
PDB entry 4rnu coloured by chain, side view.
PDB entry 4rnu coloured by chain, top view.
Source organism: Saccharomyces pastorianus
Primary publication:
STRUCTURAL AND FUNCTIONAL CONSEQUENCES OF CIRCULAR PERMUTATION ON THE ACTIVE SITE OF OLD YELLOW ENZYME.
Daugherty AB, Horton JR, Cheng X, Lutz S
OpenAccess logo ACS Catal 5 892-899 (2015)
PMID: 25692074

Function and Biology Details

Reaction catalysed: 
NADPH + acceptor = NADP(+) + reduced acceptor
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169780 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NADPH dehydrogenase 1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 400 amino acids
Theoretical weight: 44.94 KDa
Source organism: Saccharomyces pastorianus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q02899 (Residues: 2-302; Coverage: 75%)
Gene name: OYE1
Sequence domains: NADH:flavin oxidoreductase / NADH oxidase family

Ligands and Environments


Cofactor: Ligand FMN 4 x FMN
1 bound ligand:
Ligand PO4 4 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-BM
Spacegroup: P1
Unit cell:
a: 45.662Å b: 87.642Å c: 113.572Å
α: 69.32° β: 82.56° γ: 90.02°
R-values:
R R work R free
0.248 0.247 0.283
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Redesign of water networks for efficient biocatalysis.
Fink et al. (2017)
5 other articles

1 mention without citation

STRUCTURAL AND FUNCTIONAL CONSEQUENCES OF CIRCULAR PERMUTATION ON THE ACTIVE SITE OF OLD YELLOW ENZYME.
Daugherty et al. (2015)