› 4rnw

X-ray diffraction
1.55Å resolution

Truncated version of the G303 Circular Permutation of Old Yellow Enzyme

Released:
DOI: 10.2210/pdb4rnw/pdb
PDB entry 4rnw coloured by chain, front view.
PDB entry 4rnw coloured by chain, side view.
PDB entry 4rnw coloured by chain, top view.
Source organism: Saccharomyces pastorianus
Primary publication:
STRUCTURAL AND FUNCTIONAL CONSEQUENCES OF CIRCULAR PERMUTATION ON THE ACTIVE SITE OF OLD YELLOW ENZYME.
Daugherty AB, Horton JR, Cheng X, Lutz S
OpenAccess logo ACS Catal 5 892-899 (2015)
PMID: 25692074

Function and Biology Details

Reaction catalysed: 
NADPH + acceptor = NADP(+) + reduced acceptor
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169780 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NADPH dehydrogenase 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 386 amino acids
Theoretical weight: 43.38 KDa
Source organism: Saccharomyces pastorianus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q02899 (Residues: 2-292; Coverage: 73%)
Gene name: OYE1
Sequence domains: NADH:flavin oxidoreductase / NADH oxidase family

Ligands and Environments


Cofactor: Ligand FMN 2 x FMN
2 bound ligands:
Ligand EPE 2 x EPE
Ligand EDO 20 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P21
Unit cell:
a: 64.247Å b: 73.002Å c: 84.742Å
α: 90° β: 101.27° γ: 90°
R-values:
R R work R free
0.195 0.194 0.231
Expression system: Escherichia coli

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Citations

1 review citation

Redesign of water networks for efficient biocatalysis.
Fink et al. (2017)
5 other articles

2 mentions without citation

Genome and cancer single nucleotide polymorphisms of the human NEIL1 DNA glycosylase: activity, structure, and the effect of editing.
Prakash et al. (2014)
1 more