› 4tkm

X-ray diffraction
2.67Å resolution

Crystal structure of NADH-dependent reductase A1-R' complexed with NAD

Released:
DOI: 10.2210/pdb4tkm/pdb
PDB entry 4tkm coloured by chain, front view.
PDB entry 4tkm coloured by chain, side view.
PDB entry 4tkm coloured by chain, top view.
Source organism: Sphingomonas sp. A1
Primary publication:
Structure-based conversion of the coenzyme requirement of a short-chain dehydrogenase/reductase involved in bacterial alginate metabolism.
Takase R, Mikami B, Kawai S, Murata K, Hashimoto W
J Biol Chem 289 33198-214 (2014)
PMID: 25288804

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-100446 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NADH-dependent reductase for 4-deoxy-L-erythro-5-hexoseulose uronate Chains: A, B
Molecule details ›
Chains: A, B
Length: 258 amino acids
Theoretical weight: 27.37 KDa
Source organism: Sphingomonas sp. A1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A075B5H4 (Residues: 1-258; Coverage: 100%)
Gene name: a1-R'
Sequence domains: Enoyl-(Acyl carrier protein) reductase
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments

2 bound ligands:
Ligand SO4 5 x SO4
Ligand NAD 1 x NAD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL38B1
Spacegroup: P3121
Unit cell:
a: 87.898Å b: 87.898Å c: 139.821Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.199 0.195 0.278
Expression system: Escherichia coli BL21(DE3)

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Citations

6 review citations

The flexible feedstock concept in Industrial Biotechnology: Metabolic engineering of Escherichia coli, Corynebacterium glutamicum, Pseudomonas, Bacillus and yeast strains for access to alternative carbon sources.
Wendisch et al. (2016)
5 more

3 mentions without citation

Structure-based conversion of the coenzyme requirement of a short-chain dehydrogenase/reductase involved in bacterial alginate metabolism.
Takase et al. (2014)
2 more