› 4tmb

X-ray diffraction
1.8Å resolution

CRYSTAL STRUCTURE of OLD YELLOW ENZYME from CANDIDA MACEDONIENSIS AKU4588

Released:
DOI: 10.2210/pdb4tmb/pdb
PDB entry 4tmb coloured by chain, front view.
PDB entry 4tmb coloured by chain, side view.
PDB entry 4tmb coloured by chain, top view.
Source organism: Kluyveromyces marxianus
Primary publication:
An engineered old yellow enzyme that enables efficient synthesis of (4R,6R)-Actinol in a one-pot reduction system.
Horita S, Kataoka M, Kitamura N, Nakagawa T, Miyakawa T, Ohtsuka J, Nagata K, Shimizu S, Tanokura M
Chembiochem 16 440-5 (2015)
PMID: 25639703

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-179750 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NADH:flavin oxidoreductase/NADH oxidase N-terminal domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 403 amino acids
Theoretical weight: 45.95 KDa
Source organism: Kluyveromyces marxianus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6I7B7 (Residues: 1-403; Coverage: 100%)
Gene name: oye
Sequence domains: NADH:flavin oxidoreductase / NADH oxidase family
Structure domains: Aldolase class I

Ligands and Environments

1 bound ligand:
Ligand FMN 4 x FMN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NW12A
Spacegroup: C2
Unit cell:
a: 287.51Å b: 59.62Å c: 100.29Å
α: 90° β: 109.89° γ: 90°
R-values:
R R work R free
0.173 0.172 0.21
Expression system: Escherichia coli

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Citations

2 review citations

What are the Limitations of Enzymes in Synthetic Organic Chemistry?
Reetz MT. (2016)
1 more